Chromatogram Trace Viewers
4Peaks is a program that helps molecular biologists to visualize and edit their DNA sequence files. At last there is a decent solution for analyzing trace files on the Mac OS X platform, taking another step away from slow and non-native programs from the Mac OS 9 period.
- FinchTV - A Brilliant Trace Viewer. Download Review Comments (1) Questions & Answers (1) We do not have a download file for the latest version (1.5), but you can try downloading it from the developer's site.
- We are making FinchTV freely available here as a service to the community. FinchTV does not collect location or usage data. Download FinchTV 1.5 for MacOS X.If you have problems installing FinchTV on a Mac, we have some helpful hints below. Download FinchTV 1.4.0 for Windows.
These programs are limited in scope but serve a good purpose in evaluating the quality of data produced by allowing users to view the raw data associated with .ab1 files.
FinchTV (Windows/OSX)
From the makers of our data management system. Displays quality scores and much more. We really like the feature that links directly to NCBI for a BLAST search! What is more the application is FREE!
From the makers of our data management system. Displays quality scores and much more. We really like the feature that links directly to NCBI for a BLAST search! What is more the application is FREE!
4Peaks (OSX Only)
Written to run natively on OSX and makes full use of the OSX interface. The application has lots of features and is more than just a “chromatogram viewer”. The best feature is the application is totally FREE! The same people have other OSX applications that might be useful for scientists.
Written to run natively on OSX and makes full use of the OSX interface. The application has lots of features and is more than just a “chromatogram viewer”. The best feature is the application is totally FREE! The same people have other OSX applications that might be useful for scientists.
ABI Seq Scape(Windows)
Another free viewer. This program is provided by the maker of the machine we run your samples on.
Another free viewer. This program is provided by the maker of the machine we run your samples on.
Chromas LITE (Windows Only)
This application will allow PC users to display, edit and print Applied Biosystems chromatogram files. You can even translate the sequence and reverse complement it! There is a ‘Pro’ version with more features.
This application will allow PC users to display, edit and print Applied Biosystems chromatogram files. You can even translate the sequence and reverse complement it! There is a ‘Pro’ version with more features.
Traceviewer(Windows/OSX/Linux)
This is a Java program that allows the reading of sequence traces from ABI or SCF files, the display of traces on white or black background and the display of Phred quality values as bars or numbers with one of three different color schemes. TraceViewer is free for academic users. Traceviewer for the Macintosh does not suffer from the same problem as Editview.
This is a Java program that allows the reading of sequence traces from ABI or SCF files, the display of traces on white or black background and the display of Phred quality values as bars or numbers with one of three different color schemes. TraceViewer is free for academic users. Traceviewer for the Macintosh does not suffer from the same problem as Editview.
Contig Assembly and Sequence Alignment
These software packages can be used to view, edit, assemble, align, BLAST and even design primers. Some of the programs have phylogenetic analysis software built in as well! For more information, click on the links below.
Proprietary Software:
Proprietary software may cost more, but it also tends to be more current and offers long term and ongoing support.
- CLCbio DNA Workbench (Windows/OSX/Linux) Available for use on workstations in our facility
- Bioinformatics Toolbox 3.1 for MATLAB (Windows/OSX/Linux)
- CodonCode Aligner (Windows/OSX)
- Geneious(Windows/OSX/Linux)
- iNquiry (OSX Server/Unix/Linux)
- Lasergene (Windows/OSX)
- Sequencher (Windows/OSX) v4.2 available on Mac workstations in our facility
Free and Open Source Software:
Free, customizable and often times you can examine the source code. These can also be difficult to find support for.
- BioEdit (Windows only)
- EMBOSS (OSX/Unix/Linux)
- MEGA4 (Windows/OSX/Linux)
- Gene Construction Kit (Windows/OSX)
- MacVector (OSX only)
- Peaks (Windows/OSX/Linux)
- Unipro UGENE (Windows/OSX/Linux)
Analysis Software geared toward the interests of Evolutionary Analysis, Population Genetics, Phylogenetics and the like:
A List of Phylogeny Programs by Joe Felsenstein If you can’t find what you’re looking for in this comprehensive and exhaustive list, it probably doesn’t exist.
JMATING a software for the analysis of sexual selection and sexual isolation effects from mating frequency data.
Mesquite is software for evolutionary biology, designed to help biologists analyze comparative data about organisms. Its emphasis is on phylogenetic analysis, but some of its modules concern population genetics, while others do non-phylogenetic multivariate analysis.
TREEFINDER is a software to compute phylogenetic trees from molecular sequences.
TreeRot is a very easy program to use for determining Bremer indices (decay indices) for phylogenetic trees. It is also capable of providing partitioned DI, and after your analysis is complete, the program can parse your output log and give you the DI at each node (so you don’t have to go through the log file yourself and do the arithmetic).
SGRunner SG Runner is a graphical user interface for Seq-Gen.
Cadence Cadence helps to conduct Bayesian relative rates tests.
PolypSizer A program for measuring things. Very useful when combined with a digitizing pad and drawing tube.
Gel Reader A program for estimating band sizes on electrophoretic gels.
For Windows:
Acquity 4.0 (ccg12 and ccg13) | MrBayes 3.0b4 |
Clustalx 1.83 | PAUP 4.0 |
DnaSP 4.0 | Phylip 3.65 |
GenePix Pro 6.0 (ccg12 and ccg13) | Phyml 2.4.4 |
Genespring 7.2 (ccg12 and ccg13) | Seq Scape 2.1.1 |
Mega 2 | Sequencing Analysis 5.1.1 |
Mega 3.1 | TreeView |
For Macs:
Clustalx 1.83.1 |
MacClade 4.06 |
MrBayes 3.1.2 |
PAUP 4.0 |
Phylip 3.65 |
Phyml 2.4.4 |
TreeView 1.6.6 |
CoMET (Continuous-character Model Evaluation and Testing) is a Mesquite module for calculating the likelihood of different Brownian motion-based models of continuous character change on phylogenies
Modeltest A tool to select the best-fit model of nucleotide substitution
MrAIC Perl script for selecting DNA substitution models using PHYML
MrModeltest C program for selecting DNA substitution models using PAUP Mailfit: Perl script that runs PAUP and MrModeltest2 (or Modeltest) and sends (optionally) results via e-mail
Burntrees Perl script for manipulating MrBayes tree and parameter files. The script comes with an extra tool, catmb.pl, for concatenating files from separate MrBayes runs.
Vim Nexus syntax highlighting Nexus syntax highlighting files for the Vim editor. Recognizes many/most of the PAUP/MrBayes commands
Vim Nexus syntax highlighting Vim highlighting files for DNA sequence alignments
MrModelfit/Modelfit Perl script for running MrModeltest2/Modeltest
BootPHYML Perl script for performing non-parametric bootstrapping using PHYML
The .ab1 files can be viewed using the following programs for Mac and PC:
Finch TV for PC and MAC OSX is available at:
Editview for Macintosh OS9 or lower is available at:
Finchtv Dna
4 peaks for Macintosh OSX is available at:
Finchtv Download For Mac
http://www.nucleobytes.com/
Options for PC are available at:
Chromas is avalable at:
Sequence Scanner Software v1.0 is available at:
For Windows 8:
SnapGene Viewer is available at:
Acaclone pDRAW32 is available at:
Geospiza Finchtv
These programs download as self-extracting files -- download them to your computer and double click on the icon to start the extraction process